The designed enzymes might be useful as non-immunogenic alternatives in enzyme replacement therapy for treatment of lysosomal storage disorders such as Fabry disease. Ramachandran Plot Linien energetisch bevorzugt (rot referenziert spezielle sekundäre Strukturelemente) Ausserhalb der Linie nicht erlaubt Die meisten Aminosäuren fallen in R Regionen (right-handed alpha helix) oder Regionen (beta-strand) Glycine hat zusätzliche Konformationen (e.g. Comparison of the crystal structures of the designed enzyme to the wild-type enzymes shows that active sites superimpose well, indicating success of the rational design. As a result, I-TASSER models sometime have more backbone torsion angles within energetically unfavorable regions in the Ramachandran plot this happens more often for the difficult targets that have no close homologous templates. Conversely, the engineered α-NAGAL retains the antigenicity but has acquired the enzymatic specificity of the α-GAL enzyme. The engineered α-GAL retains the antigenicity but has acquired the enzymatic specificity of α-NAGAL.
Using a rational protein engineering approach, we interconverted the enzymatic specificity of α-GAL and α-NAGAL.
The human lysosomal enzymes α-galactosidase and α-N-acetylgalactosaminidase share 46% amino acid sequence identity and have similar folds. Predictions of the local quality (per-residue error) within multiple models. In this case, the pump receives increased electrical power, but the mechanical strength of the pump is (relatively) reduced. problems with the readings of LP sensor b. Rankings for multiple 3D models for the same protein target according to predicted model quality. For the existing (this is a typical/widespread problem in the case of 2AAF) situation, two scenarios are possible: a.
IB Tomasic, MC Metcalf, AI Guce, NE Clark, SC Garman. The ModFOLD8 server provides: A single score and a p-value relating to the predicted quality of a single 3D model of a protein structure. Interconversion of the specificities of human lysosomal enzymes associated with Fabry and Schindler diseases.